\u7b2c53\u56de\u751f\u7269\u6a5f\u68b0\u30b7\u30b9\u30c6\u30e0\u7814\u7a76\u4f1a
\uff08\u5927\u962a\u5927\u5b66\u5927\u5b66\u9662\u57fa\u790e\u5de5\u5b66\u7814\u7a76\u79d1\u6a5f\u80fd\u5275\u6210\u5c02\u653b \u5171\u50ac\uff09
\u3010\u65e5\u6642\u30112020\u5e7412\u67088\u65e5\uff08\u706b\uff09\u5348\u524d9:30-10:30
\u3010\u5834\u6240\u3011\u30aa\u30f3\u30e9\u30a4\u30f3\u958b\u50ac
\u3010\u53c2\u52a0\u8005\u306e\u7686\u69d8\u3078\u306e\u304a\u9858\u3044\u3011
\u5f53\u65e5\u306fZoom\u30df\u30fc\u30c6\u30a3\u30f3\u30b0\u306b\u5165\u5ba4\u5f8c\uff0c\u30c1\u30e3\u30c3\u30c8\u753b\u9762\u306b\u6c0f\u540d\u3068\u6240\u5c5e\u306e\u8a18\u5165\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\uff0e
\u3010\u8b1b\u6f14\u8005\u30fb\u8b1b\u6f14\u984c\u76ee\u30fb\u8b1b\u6f14\u8981\u65e8\u3011
————————————————
\u8b1b\u6f141\uff089:30-10:00\uff09
Cristina Bertocchi (Department of Physiology, Pontificia Universidad Cat\u00f3lica de Chile)
\u201c Mechano-molecular mechanisms regulating cell adhesion nanomachines “
Cells sense their physical surroundings through molecular nanomachines regulating force transduction and mechanosensing. One of such complexes is the Adherens Junction (AJ), an adhesion complex mediating cell-cell interaction. Within AJ, the protein a-catenin, has been suggested as a force-transducing element. However, it is unlikely that a single dominant tether could bear the load of and regulate such sophisticated and precise system to provide the high morphological plasticity observed in epithelial tissues. Indeed, we have previously unveiled a mechanism by which conformational switch of vinculin (a partner protein of a-catenin)
could selectively engage the actin cytoskeleton in response to regulatory mechanochemical signals, effectively functioning as a molecular clutch, to mediate intercellular interactions. This finding implies that the force transduction and regulation at AJ may be more complex than a single tether functioning through a simple on-off model. Using a super-resolution microscopy approach, we report that vinculin, once activated, could form a direct structural connection with b-catenin, which can bypass a-catenin. Direct vinculin\/b-catenin interaction can support mechanical tension and contributes to the stabilization of the cadherin-catenin complexes. We thus propose a multi-step mechanochemical process to mediate mechanical connection and the force-dependent molecular mechanics modulating AJ reinforcement in complex biological functions.
————————————————
\u8b1b\u6f142\uff0810:00-10:30\uff09
Hiroaki Hirata (\u5e73\u7530 \u5b8f\u8061) (Graduate School of Medicine, Nagoya University; \u540d\u53e4\u5c4b\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7cfb\u7814\u7a76\u79d1)
\u201c Molecular and biophysical bases for modulation of the focal adhesion-actin cytoskeleton system in response to extracellular substrate rigidity “
Rigidity of the extracellular substrate largely influences cell adhesion and morphogenesis. On soft substrates, cells cannot stabilize cell-substrate adhesion, resulting in a failure of cell spreading on the substrates. While cell-substrate adhesion is primarily mediated by focal adhesions, their stabilization depends on formation of the stable linkage of the actin cytoskeleton with the focal adhesions. Here, we propose a potential mechanism for rigidity-dependent regulation of the actin cytoskeleton-focal adhesion linkage. Talin molecules linking between the retrograding actin cytoskeleton and the adhesion receptor integrin at focal adhesions are mechanically extended in a manner dependent on the extracellular substrate rigidity. Even though the talin-actin bond is short-lived, extension of talin exposes cryptic binding sites for the actin-binding protein vinculin, causing reinforcement of the talin-actin bond by vinculin. Once cell adhesion is stabilized, phosphorylation of another actin-binding protein calponin 3 regulates contractile force generation by actomyosin in response to rigidity of the extracellular substrate. Rigid substrates promote calponin 3 phosphorylation by MEKK1, which increases actomyosin-based generation of contractile force. Thus, modulation at multiple layers of the focal adhesion-actin cytoskeleton system would underlie proper responses of cell adhesion and morphology against rigidity of the extracellular substrate.<\/p>\n
—————————————————————<\/p>\n","protected":false},"excerpt":{"rendered":"
\u7b2c53\u56de\u751f\u7269\u6a5f\u68b0\u30b7\u30b9\u30c6\u30e0\u7814\u7a76\u4f1a\uff08\u5927\u962a\u5927\u5b66\u5927\u5b66\u9662\u57fa\u790e\u5de5\u5b66\u7814\u7a76\u79d1\u6a5f\u80fd\u5275\u6210\u5c02\u653b \u5171\u50ac\uff09\u3010\u65e5\u6642\u30112020\u5e7412\u67088\u65e5\uff08\u706b\uff09\u5348\u524d9:30-10:30\u3010\u5834\u6240\u3011\u30aa\u30f3\u30e9\u30a4\u30f3\u958b\u50ac\u3010\u53c2\u52a0\u8005\u306e\u7686\u69d8\u3078\u306e\u304a\u9858\u3044\u3011\u5f53\u65e5\u306fZoom\u30df\u30fc\u30c6\u30a3\u30f3\u30b0\u306b\u5165\u5ba4\u5f8c […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[26],"class_list":["post-4151","post","type-post","status-publish","format-standard","hentry","category-news","tag-26"],"_links":{"self":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/posts\/4151","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4151"}],"version-history":[{"count":0,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/posts\/4151\/revisions"}],"wp:attachment":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4151"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4151"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4151"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}