{"id":6631,"date":"2025-03-03T19:30:52","date_gmt":"2025-03-03T10:30:52","guid":{"rendered":"https:\/\/mbm.me.es.osaka-u.ac.jp\/?p=6631"},"modified":"2025-03-03T19:39:46","modified_gmt":"2025-03-03T10:39:46","slug":"%e7%99%ba%e8%a1%a8%e8%ab%96%e6%96%87%e8%a7%a3%e8%aa%ac%ef%bc%9a%e3%82%b9%e3%83%88%e3%83%ac%e3%82%b9%e3%83%95%e3%82%a1%e3%82%a4%e3%83%90%e3%83%bc%e3%82%92%e6%a7%8b%e6%88%90%e3%81%99%e3%82%8b%e5%85%a8","status":"publish","type":"post","link":"https:\/\/mbm.me.es.osaka-u.ac.jp\/?p=6631","title":{"rendered":"\u767a\u8868\u8ad6\u6587\u89e3\u8aac\uff1a\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\u3092\u69cb\u6210\u3059\u308b\u5168\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u540c\u5b9a\uff1a\u7d30\u80de\u8001\u5316\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u8feb\u308b"},"content":{"rendered":"<p><span style=\"font-size: 14pt;\"><strong>Proteome of actin stress fibers<\/strong><\/span><\/p>\n<p>Stress fibers are cytoskeletal structures primarily composed of actin filaments and non-muscle myosin II, which play a crucial role in maintaining cellular architecture and mediating adhesion to surrounding tissues. Stress fibers function as key mechanosensors, responding to the rigidity of the extracellular matrix and external mechanical stimuli. A defining feature of these structures is their dynamic reorganization, which enables cells to adapt to environmental changes.<\/p>\n<p>In this study (Liu et al., Mol. Biol. Cell 33, 2022), we identified the constituent proteins of stress fibers and investigated their expression changes during cellular senescence. First, using a previously developed method (<a href=\"https:\/\/royalsocietypublishing.org\/doi\/full\/10.1098\/rsfs.2011.0031?casa_token=jJwtmcEUzroAAAAA%3Ajel3S5OHa8Pojq-xOrZIQIxwaEi7zjSsw4FqPa4rWImEUtsmg1T-b0wRK0HGysiuSRBxFBNUrnoTqg\">1<\/a>, <a href=\"https:\/\/link.springer.com\/article\/10.1007\/s10237-019-01228-z\">2<\/a>, etc.), we isolated stress fibers from human fibroblasts and conducted proteomic analysis to characterize their protein composition. We then performed a comprehensive analysis of factors whose expression levels change as cells undergo senescence.<\/p>\n<p>Among these, we focused on elongation factor 2 (eEF2) and found that its expression increases along the length of stress fibers during cellular senescence. We demonstrated that this upregulation leads to a delayed progression in cell proliferation, migration, and metabolic activity. Thus, our study provides a molecular-level understanding of the relationship between cellular senescence and the actin cytoskeleton, highlighting a key mechanism of senescence-associated functional decline in cells.<\/p>\n<p><span style=\"font-size: 14pt;\"><strong>\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\u306e\u30d7\u30ed\u30c6\u30aa\u30fc\u30e0<\/strong><\/span><\/p>\n<p>\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\uff08stress Fibers\uff09\u306f\u3001\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3068\u975e\u7b4bII\u578b\u30df\u30aa\u30b7\u30f3\u3092\u4e3b\u6210\u5206\u3068\u3059\u308b\u7d30\u80de\u9aa8\u683c\u69cb\u9020\u3067\u3042\u308a\u3001\u7d30\u80de\u306e\u69cb\u9020\u7dad\u6301\u3084\u5468\u56f2\u7d44\u7e54\u3068\u306e\u63a5\u7740\u306a\u3069\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u307e\u3059\u3002\u7279\u306b\u3001\u7d30\u80de\u304c\u7d30\u80de\u5916\u57fa\u8cea\u306e\u525b\u6027\u3084\u5916\u90e8\u304b\u3089\u306e\u529b\u5b66\u7684\u523a\u6fc0\u3092\u611f\u77e5\u30fb\u5fdc\u7b54\u3059\u308b\u969b\u306b\u6a5f\u80fd\u3057\u3001\u74b0\u5883\u306e\u5909\u5316\u306b\u9069\u5fdc\u3059\u308b\u305f\u3081\u306b\u305d\u306e\u69cb\u9020\u3084\u914d\u7f6e\u3092\u52d5\u7684\u306b\u5909\u5316\u3055\u305b\u308b\u3053\u3068\u304c\u7279\u5fb4\u3067\u3059\u3002<\/p>\n<p>\u672c\u8ad6\u6587\uff08Liu et al., Mol. Biol. Cell 33, 2022\uff09\u3067\u306f\u3001\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\u306e\u69cb\u6210\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u8001\u5316\u306b\u4f34\u3046\u767a\u73fe\u5909\u5316\u3092\u8abf\u3079\u307e\u3057\u305f\u3002\u307e\u305a\u3001\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\u3092\u3001\u904e\u53bb\u306b\u958b\u767a\u3057\u305f\u65b9\u6cd5\uff08<a href=\"https:\/\/royalsocietypublishing.org\/doi\/full\/10.1098\/rsfs.2011.0031?casa_token=jJwtmcEUzroAAAAA%3Ajel3S5OHa8Pojq-xOrZIQIxwaEi7zjSsw4FqPa4rWImEUtsmg1T-b0wRK0HGysiuSRBxFBNUrnoTqg\">1<\/a>, <a href=\"https:\/\/link.springer.com\/article\/10.1007\/s10237-019-01228-z\">2<\/a>\u306a\u3069\uff09\u306b\u3088\u3063\u3066\u7dda\u7dad\u82bd\u7d30\u80de\u304b\u3089\u5358\u96e2\u3057\u3001\u305d\u306e\u30d7\u30ed\u30c6\u30aa\u30fc\u30e0\u89e3\u6790\u306b\u3088\u308a\u69cb\u6210\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\uff08\u4e0b\u56f3\uff09\u3002\u3055\u3089\u306b\u3001\u7d30\u80de\u8001\u5316\u306b\u4f34\u3044\u767a\u73fe\u91cf\u304c\u5909\u5316\u3059\u308b\u56e0\u5b50\u3092\u7db2\u7f85\u7684\u306b\u89e3\u6790\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u7279\u306beEF2\uff08elongation factor 2\uff09\u306b\u7740\u76ee\u3057\u3001\u7d30\u80de\u8001\u5316\u306b\u4f34\u3044\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\u306b\u6cbf\u3063\u3066\u767a\u73fe\u91cf\u304c\u4e0a\u6607\u3059\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u3053\u306e\u5897\u52a0\u304c\u7d30\u80de\u306e\u5897\u6b96\u3001\u904a\u8d70\u3001\u4ee3\u8b1d\u901f\u5ea6\u3092\u9045\u5ef6\u3055\u305b\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306b\u672c\u7814\u7a76\u306f\u3001\u7d30\u80de\u8001\u5316\u3068\u7d30\u80de\u9aa8\u683c\u306e\u95a2\u4fc2\u3092\u5206\u5b50\u30ec\u30d9\u30eb\u3067\u89e3\u660e\u3057\u3001\u8001\u5316\u306b\u4f34\u3046\u7d30\u80de\u6a5f\u80fd\u306e\u4f4e\u4e0b\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u4e00\u7aef\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u73fe\u8c61\u30ec\u30d9\u30eb\u306e\u89b3\u5bdf\u306b\u7559\u307e\u308a\u304c\u3061\u3067\u3042\u3063\u305f\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\u306b\u95a2\u3059\u308b\u7814\u7a76\u3092\u5206\u5b50\u30ec\u30d9\u30eb\u3067\u89e3\u660e\u3059\u308b\u9053\u3092\u62d3\u3044\u305f\u70b9\u3067\u610f\u7fa9\u6df1\u3044\u3082\u306e\u3068\u4f4d\u7f6e\u4ed8\u3051\u3066\u3044\u307e\u3059\u3002\u30b9\u30c8\u30ec\u30b9\u30d5\u30a1\u30a4\u30d0\u30fc\u306f\u3001\u7d30\u80de\u306e\u69cb\u9020\u5909\u5316\u306b\u5fdc\u3058\u3066\u9069\u5fdc\u7684\u306b\u518d\u69cb\u7bc9\u3055\u308c\u308b\u305f\u3081\u3001\u6211\u3005\u304c\u95a2\u5fc3\u3092\u3082\u3064\u751f\u547d\u306e\u5358\u4f4d\u30fb\u7d30\u80de\u306e\u74b0\u5883\u9069\u5fdc\u306e\u7269\u7406\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u7a76\u660e\u3092\u4e00\u5c64\u9032\u3081\u308b\u3046\u3048\u3067\u3082\u91cd\u8981\u306a\u77e5\u898b\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002<\/p>\n<p><a href=\"https:\/\/mbm.me.es.osaka-u.ac.jp\/wp-content\/uploads\/2025\/03\/9343a951c35918167dc9c5bc1c3dc07f-scaled.jpg\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-large wp-image-6633\" src=\"https:\/\/mbm.me.es.osaka-u.ac.jp\/wp-content\/uploads\/2025\/03\/9343a951c35918167dc9c5bc1c3dc07f-1500x1060.jpg\" alt=\"\" width=\"800\" height=\"565\" srcset=\"https:\/\/mbm.me.es.osaka-u.ac.jp\/wp-content\/uploads\/2025\/03\/9343a951c35918167dc9c5bc1c3dc07f-1500x1060.jpg 1500w, https:\/\/mbm.me.es.osaka-u.ac.jp\/wp-content\/uploads\/2025\/03\/9343a951c35918167dc9c5bc1c3dc07f-300x212.jpg 300w, https:\/\/mbm.me.es.osaka-u.ac.jp\/wp-content\/uploads\/2025\/03\/9343a951c35918167dc9c5bc1c3dc07f-1536x1085.jpg 1536w, https:\/\/mbm.me.es.osaka-u.ac.jp\/wp-content\/uploads\/2025\/03\/9343a951c35918167dc9c5bc1c3dc07f-2048x1447.jpg 2048w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Proteome of actin stress fibers Stress fibers are cytoskeletal structures primarily composed of actin filament [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-6631","post","type-post","status-publish","format-standard","hentry","category-1"],"_links":{"self":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/posts\/6631","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6631"}],"version-history":[{"count":0,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=\/wp\/v2\/posts\/6631\/revisions"}],"wp:attachment":[{"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6631"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6631"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mbm.me.es.osaka-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6631"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}